Homepage - The homepage displays options for different modules available for the user for independent analysis. Users can click on the module icons to proceed further.
Offline PBIT - Users can choose the offline PBIT option from the menu bar to download the offline version of the tool. Details are given on the “offline PBIT” page.
Build pipeline - The "Build Pipeline" section is the main feature of the website. It allows users to create custom pipelines for target identification. The pipeline building process consists of the following steps:
Step 1: Select Modules - Users can choose the modules (and their submodules) they want to include in their pipeline. The available modules are listed on the website, and users can select multiple modules for their pipeline.
Step 2: Run Pipeline – Using the arrows, the modules can be arranged according to the order you want to execute each module. Once the pipeline is built, users can run it by clicking the "Submit" button. The website will process the input data using the selected tools and generate the output.
Step 3: Input Parameters - Users can execute the selected modules by providing the necessary input parameters. The green highlighted box indicates the current module being executed.
Step 4: Download Results - Once executed, the results can be downloaded and saved on the system, and the next module can be executed.
KEY:
Module name
Input File
Parameters
Output
Screening & Characterization
FASTA formatted protein sequences
Choice of submodules Percent identity Alignment length cut off E-value
Displays the number of homologous/non-homologous hits obtained against database with input parameters.
The BLAST output can be downloaded and saved.
Druggability Analysis
FASTA formatted protein sequences
Percent identity E-value
Displays table containing FASTA formatted protein sequences with the number of hits and blast reports.
Immunoinformatics Analysis
FASTA formatted protein sequences
Choice of methods Window Size Alleles
Displays table containing the peptides, positions, and their respective scores as the output of selected modules.
Systems biology Analysis
Metabolic model in JSON format Edge list in CSV format (Network Analysis) Gene list from model (optional)
Choice of submodules
Reaction ids & bounds (optional)
Displays table containing the genes or proteins with their respective fluxes/growth rate/score as the output of selected modules.
